University of Heidelberg
BIOQUANT

ViroQuant-CellNetworks RNAi Screening Facility

Sample preparation

 

"ready to transfect" siRNAs → "ready for DAQ" assays

 

With fluorescent proteins tagged cDNAs or small interfering siRNAs are used for solid phase reverse transfection on well plates or cell arrays. This is a high-throughput method for the parallel transfection of mammalian cells:

  1. Preparation of transfection solution
  2. Solid phase reverse transfection on cell arrays or well plates

Finally, tissue culture cells are plated on the dried slides resulting in clusters of live cells with a specific gene perturbation in a lawn of untransfected cells ("reverse transfection") and, depending on the biological approach, prepared for further analysis (fixation or live cell imaging).

 

 

1. Preparation of transfection solution
2. Solid phase reverse transfection

Pipetting robot
96er pipetting head

Pipetting robot

Hamilton "STAR"

 

Automated preparation of transfection solution in 384er source plates

 

  • The labware carriers can be equipped with standard well plate formats

Contact printer
384er print head

Contact printer [384er print head]

"Graffinity"

 

Printing of transfection solution on up to "genome wide" cell arrays

 

  • Solid phase reverse transfection onto glass slides   [Microarraying with ø(spots) ~400µm]

Contact printer
Spotting process

Contact printer [8er print head]

Bio-Rad "Chipwriter Pro"

 

Printing of transfection solution on up to 384er cell arrays

 

  • Solid phase reverse transfection onto glass slides   [Microarraying with ø(spots) ~400µm]

'SpeedTrap'

'Speed Trap'

miVac "Modular Concentrator"

 

Application of transfection solution to well plates

 

  • Solid phase reverse transfection on standard well plate formats
Contact: E-Mail (Last update: 07/12/2011)